KMID : 0355620100360050392
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Journal of Korean Association of Oral and Maxillofacial Surgeons 2010 Volume.36 No. 5 p.392 ~ p.401
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Effect of adipose-derived stem cells on bone healing on titanium implant in tibia of diabetes mellitus induced rats
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Kim Min-Gu
Jung In-Kyo Shin Sang-Hun Kim Chul-Hoon Kim Bok-Joo Kim Jung-Han Hwang Young-Sup Jung Eu-Gene Kim Jin-Woo Kim Uk-Kyu
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Abstract
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Introduction: Diabetes mellitus, as a major health problem for the elderly has been shown to alter the properties of the bone and impair bone healing around a titanium implant in both humans and animals. The aim of this study was to examine the effect of adipose-derived stem cells on the healing process around a titanium implant in streptozotocin-induced diabetic rats.
Materials and Methods: Thirteen rats were divided into two groups: adipose-derived stem cells injected group and a control group. A titanium screw implant (diameter: 2.0 §®, length: 3.5 §®) was placed into both tibia of 13 rats: 13 right tibia as the control group and 13 left tibia as the experimental group. The rats were sacrificed at different intervals (1, 2, and 4 weeks) after implantation for histopathology observations and i§®unohistochemistric analysis.
Results: The histopathological findings revealed earlier new formed bone in the experimental group than the control group. In particular, at 1 week after implantation, the experimental group showed more newly formed bone and collagen around the implant than the control group. In i§®unohistochemistric analysis, osteoprotegerin (OPG) expression in the experimental group increased early compared to that of the control group until 2 weeks after implantation. However, after 2 weeks, OPG expression in the experimental group was similar to OPG expression in the control group. The receptor activator of nuclear factor ¥êB ligand (RANKL) expression in the experimental group increased early compared to that of the control group, and then decreased at 2 weeks. After 2 weeks, the level of RANKL expression was similar in both groups.
Conclusion: These results suggest that adipose-derived stem cells in implantation can promote bone healing around titanium, particularly in diabetes mellitus induced animals.
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KEYWORD
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Stem cell, Dental implants, Diabetes mellitus, Rats
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